Hi, is there a way we can determine the profit and loss of a single sales order?
No.
First, a sales order is not a financial transaction, so there is no profit or loss. Sales orders have no financial impact on any account.
Second, profit and loss is a very broad concept and includes many things outside what is recorded by a single transaction. The idea would be largely meaningless, for example, for someone selling services.
This notion has been raised many times before in the forum. Search for those other discussions.
Have you tried using the Projects Feature?